Confocal microscopy manual

 · MIC guidelines mandate that the use of the Olympus Confocal Microscope must be properly acknowledged in any publication (including web pages). You can use the following statement: “The use of the Microscopy and Imaging Center facility at Texas AM University is acknowledged. The Olympus FV confocal microscope acquisition was supported by the. Confocal Laser Scanning Microscopy (LEICA) This presentation has been put together as a common effort of Urs Ziegler, Anne Greet Bittermann, Mathias Hoechli. Many pages are copied from Internet web pages or from presentations given by Leica, Zeiss and other companies. Please browse the internet to learn interactively all about optics. stack. Use the Confocal Controller, or the microscope controller fine focusing knob. Fine adjustments may be made using the clickable wheel. 2. Click on Set Start. 3. Re-adjust the focus to the bottom of the stack. 4. Click on Set End. The number of steps and the step size will be automatically computed based on the longest wavelength emission band. It.

Zeiss upright confocal manual. Capabilities: High sensitivity point scanning confocal imaging. Spectral array GaAsP detectors. Upright motorized stage microscope. In vivo or in vitro samples. Leica SP5 Inverted Confocal Manual. Capabilities: Imaging diverse fluorophores in fixed or live samples. Temperature, humidity, CO2 control. Spectrally tunable detectors. Ultra-fast resonant scanning (reduced photo-damage with live samples) 3D z-stacks, timelapse, multi-position timelapse. FRAP/FLIP/Photoactivation, FRET (ratio or acceptor. The following drawing shows a typical confocal fluorescence microscope setup. In this setup, the objective focuses the excitation light from the laser at the fluorescent sample and, at the same time, collects the resulting emission. The emission photons pass through the confocal aperture and arrive at the single-photon detector (SPD).

6 ส.ค. Confocal Microscope. User Guide o Manual coarse and fine knobs on each side Note: Fluorescence at the microscope uses the Mercury-. SP5 confocal microscope user manual, Hélène Javot updated by Mamta Srivastava 2/11/, page 1/ PCIC. Plant Cell Imaging Center. (BTI, room B). Knowledge of this manual is required for the operation of the instrument. over conventional microscopy in terms of resolving power and confocal depth.